ABSTRACT
OBJECTIVE@#To investigate the structural characteristics of the cerebral small vessels with an unknown type of pathological lesion (UTPL).@*METHODS@#Contents of beta-amyloid, alpha-actin and collagen IV in cerebral small vessels with UTPL were studied by Congo red staining, immunohistochemical staining and computer image analysis.@*RESULTS@#The low expression levels of alpha-actin and collagen IV (P<0.05) were observed in tunica media of the vessels with UTPL, and no positive expression of beta-amyloid (P>0.05) was observed in these vessel walls. The expressions of proteins mentioned above in UTPL were different from those of cerebral amyloid angiopathy(CAA) and hyaline arteriolosclerosis.@*CONCLUSION@#UTPL was different from CAA or hyaline arteriolosclerosis in pathologic feature.
Subject(s)
Humans , Actins/metabolism , Amyloid beta-Peptides/metabolism , Autopsy , Blood Vessels/ultrastructure , Brain/pathology , Cerebral Amyloid Angiopathy/pathology , Collagen Type IV/metabolism , Image Processing, Computer-Assisted , Immunohistochemistry , Staining and Labeling , Subarachnoid Hemorrhage/pathologyABSTRACT
OBJECTIVE@#To investigate the distribution of mophine in organs in cases with morphine poisioning to select ideal organs for immunohistochemical derection.@*METHODS@#Localization and half quantitation of morphine in the brain, the kidney, the heart, and the liver were studied in 8 cases with morphine poisoning by immunohistochemical SP method.@*RESULTS@#Morphine was mainly detected in the cytoplasm of certain parenchymal cells of the organs. The distribution varied greatly with different cases and organs. In the brain and kidney, morphine-positive cells could be easily found.@*CONCLUSION@#The kidney and brain may be the ideal organs for sampling in suspected morphine poisoning cases with.